365D
STRUCTURAL BASIS FOR G C RECOGNITION IN THE DNA MINOR GROOVE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-04 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.396, 38.959, 44.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26000 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.857) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.027 |
| Number of reflections | 4124 |
| Completeness [%] | 93.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 4 * | pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 4 * | pH 7.50, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 1 | 2 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 2 | 2 | 1 | MPD | ||
| 3 | 1 | 1 | CA ACETATE | ||
| 3 | 2 | 1 | CA ACETATE | ||
| 4 | 1 | 1 | TRIS | ||
| 4 | 2 | 1 | TRIS | ||
| 5 | 1 | 2 | WATER | ||
| 5 | 2 | 2 | WATER | ||
| 6 | 1 | 2 | MPD | ||
| 6 | 2 | 2 | MPD |
