Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-03-28 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 59.030, 59.030, 254.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.272 |
| Rwork | 0.272 |
| R-free | 0.32400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.090 |
| High resolution limit [Å] | 3.000 * | 2.980 |
| Rmerge | 0.047 | 0.372 |
| Number of reflections | 4950 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 82.5 | 47.5 |
| Redundancy | 3.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 * | 20 * | pH 6.40, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | NA MES | ||
| 4 | 1 | 1 | MG SULFATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2 (mM) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | MPD | 5-25 (%) | |
| 5 | 1 | reservoir | 5-25 (mM) | ||
| 6 | 1 | reservoir | sodium cacodylate | 5-25 (mM) |
