Experimental procedure
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1995-03-28 |
Detector | PRINCETON 2K |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.030, 59.030, 254.070 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 3.000 |
R-factor | 0.272 |
Rwork | 0.272 |
R-free | 0.32400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.090 |
High resolution limit [Å] | 3.000 * | 2.980 |
Rmerge | 0.047 | 0.372 |
Number of reflections | 4950 | |
<I/σ(I)> | 10.4 | 2 |
Completeness [%] | 82.5 | 47.5 |
Redundancy | 3.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 * | 20 * | pH 6.40, VAPOR DIFFUSION |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MGCL2 | ||
3 | 1 | 1 | NA MES | ||
4 | 1 | 1 | MG SULFATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.2 (mM) | |
2 | 1 | drop | 5 (mM) | ||
3 | 1 | drop | sodium cacodylate | 50 (mM) | |
4 | 1 | reservoir | MPD | 5-25 (%) | |
5 | 1 | reservoir | 5-25 (mM) | ||
6 | 1 | reservoir | sodium cacodylate | 5-25 (mM) |