35YM
Structure of an Enterococcus faecium O-glycopeptidase in complex with the T-antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-17 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.674, 82.406, 84.445 |
| Unit cell angles | 70.11, 77.28, 80.30 |
Refinement procedure
| Resolution | 27.720 - 2.280 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.466 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.102 | 0.492 |
| Rpim | 0.051 | 0.293 |
| Number of reflections | 50563 | 1817 |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.983 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M NaCl and 20% w/v PEG 3350 |
