35VU

Crystal structure of apo DUF190

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 31
Unit cell lengths	58.910, 58.910, 348.037
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.960 - 2.200
R-factor	0.1925
Rwork	0.191
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.020	2.250
High resolution limit [Å]	2.200	2.200
Rmerge		0.259
Number of reflections	68552	4659
<I/σ(I)>	13
Completeness [%]	99.9
Redundancy	8.7
CC(1/2)	0.992	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.1 M HEPES sodium pH 7.5, 10% v/v 2-propanol, 20% w/v PEG 4000