35UJ
Crystal structure of TFPI K2 domain in complex with 4D8 Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.102, 82.284, 103.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.850 - 2.900 |
| R-factor | 0.1741 |
| Rwork | 0.171 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.260 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 3.180 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.124 | 0.480 |
| Rmeas | 0.140 | 0.530 |
| Number of reflections | 12275 | 2872 |
| <I/σ(I)> | 4.47 | |
| Completeness [%] | 99.8 | 99.85 |
| Redundancy | 5.77 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12 % PEG 8000, 100 mM magnesium acetate, 100 mM MOPS pH 7.5 |
