35CF

MurJ (MviN) extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.965459
Spacegroup name	P 1 21 1
Unit cell lengths	34.134, 50.710, 73.033
Unit cell angles	90.00, 103.25, 90.00

Refinement procedure

Resolution	41.280 - 1.430
Rwork	0.174
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.575
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.126))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.280	1.481
High resolution limit [Å]	1.430	1.430
Number of reflections	59490	34163
<I/σ(I)>	1.3
Completeness [%]	93.8
Redundancy	1.7
CC(1/2)	0.575	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Ammonium Sulfate