354D
Structure of loop E FROM E. coli 5S RRNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-09-09 |
| Detector | PRINCETON 2K |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.184, 37.654, 32.944 |
| Unit cell angles | 90.00, 105.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.055 | 0.480 |
| Number of reflections | 12306 | |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 90.7 | 54 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 20 * | pH 6.00, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.2 (mM) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | MPD | 5-25 (%) | |
| 5 | 1 | reservoir | 5-25 (mM) | ||
| 6 | 1 | reservoir | sodium cacodylate | 5-25 (mM) |
