353D
CRYSTAL STRUCTURE OF DOMAIN A OF THERMUS FLAVUS 5S RRNA AND THE CONTRIBUTION OF WATER MOLECULES TO ITS STRUCTURE
Experimental procedure
| Source type | SEALED TUBE |
| Source details | CONVENTIONAL Mo |
| Temperature [K] | 153 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.7107 |
| Spacegroup name | P 43 |
| Unit cell lengths | 30.100, 30.100, 86.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.18 |
| Starting model (for MR) | NDB ENTRY ARN035 (PDB ENTRY 1RNA) |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.068 |
| Data reduction software | XDS |
| Phasing software | AMoRE |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 2477 |
| Completeness [%] | 83.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 45 * | Lorenz, S., (1993) Acta Crystallogr.,Sect.D, 49, 418. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 45 * | Lorenz, S., (1993) Acta Crystallogr.,Sect.D, 49, 418. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | MGCL2 | ||
| 8 | 1 | 2 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5 (mg/ml) | |
| 2 | 1 | drop | 5 (%) | ||
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 5 | 1 | reservoir | MPD | 30 (%) | |
| 6 | 1 | reservoir | 400 (mM) | ||
| 7 | 1 | reservoir | sodium cacodylate | 40 (mM) |
