34DD
Homo sapiens Eukaryotic translation initiation factor 4E (eIF4E) in complex with synthetic mRNA cap analogs
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2025-12-03 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.042, 128.493, 71.634 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 42.380 - 1.750 |
| R-factor | 0.20725 |
| Rwork | 0.206 |
| R-free | 0.23866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.647 |
| Data reduction software | XDS (20250714) |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.155 | 2.487 |
| Rmeas | 0.161 | 2.575 |
| Rpim | 0.042 | 0.663 |
| Total number of observations | 1023943 | 57236 |
| Number of reflections | 71323 | 3895 |
| <I/σ(I)> | 10 | 1.1 |
| Completeness [%] | 98.9 | |
| Redundancy | 14.4 | 14.7 |
| CC(1/2) | 0.999 | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 281 | 18 % w/v PEG 10,000; 20 % w/v Glycerol; 100 mM TRIS; pH 8.5; 100 mM Sodium chloride |
