336D
INTERACTION BETWEEN LEFT-HANDED Z-DNA AND POLYAMINE-3 THE CRYSTAL STRUCTURE OF THE D(CG)3 AND THERMOSPERMINE COMPLEX
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.980, 31.510, 44.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 2 | THERMOSPERMINE_HCL | ||
| 3 | 1 | 3 | MGCL2 | ||
| 4 | 1 | 4 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salt | 2 (mM) | |
| 2 | 1 | reservoir | thermospermine tetrachloride | 10 (mM) | |
| 3 | 1 | reservoir | 15 (mM) | ||
| 4 | 1 | reservoir | sodium cacodylate | 30 (mM) |
