335D
THE CRYSTAL STRUCTURE OF THE DNA DECAMER D(GGCAATTGCG) CONTAINS BOTH MAJOR AND MINOR GROOVE BINDING G.(G.C) TRIPLETS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 27.920, 39.210, 54.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.195 * |
| Rwork | 0.203 |
| Starting model (for MR) | PIECE OF UDJ031 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.800 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.080 |
| Total number of observations | 1944 * |
| Number of reflections | 1177 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 * | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 2 (mM) | |
| 2 | 1 | drop | 58 (mM) | ||
| 3 | 1 | drop | MPD | 5 (%) | |
| 4 | 1 | reservoir | MPD | 30 (%) | pH7.0 |
