331D
CRYSTAL STRUCTURE OF D(GCGCGCG) WITH 5'-OVERHANG G'S
Experimental procedure
| Source type | ROTATING ANODE |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-11 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 20.410, 29.650, 51.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.650 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Z-DNA HEXAMER D(CGCGCG) |
| RMSD bond length | 0.020 |
| RMSD bond angle | 3.200 |
| Data reduction software | RIGAKU |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.800 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.066 | 0.130 |
| Total number of observations | 9304 * | |
| Number of reflections | 3813 | |
| <I/σ(I)> | 5 | 5 |
| Completeness [%] | 92.3 | 86.5 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | SPERMINE_HCL | ||
| 4 | 1 | 1 | [CO(NH3)6]3+ | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | ISOPROPANOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | single strand concentration |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | spermine tetrachloride | 0.5 (mM) | |
| 4 | 1 | reservoir | 2-propanol | 10 (%) |
