32RM
Crystal structure of the human SPRY domain-containing SOCS box protein SPSB4 - Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-12-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.740, 83.802, 68.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 - 1.550 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.900 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rpim | 0.095 | 1.272 |
| Number of reflections | 26844 | 1300 |
| <I/σ(I)> | 7.6 | 0.7 |
| Completeness [%] | 100.0 | 98.8 |
| Redundancy | 13.2 | 9.8 |
| CC(1/2) | 0.997 | 0.342 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Sodium Citrate pH 5.5, 20% PEG3000 |
