32RD
Crystal structure of YTHDF2 in complex with compound 3 (FE468)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.380, 80.380, 113.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.870 - 1.550 |
| R-factor | 0.1992 |
| Rwork | 0.198 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1.5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.870 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 59193 | 9403 |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 8.8 | |
| CC(1/2) | 1.000 | 0.801 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2 |
