32MD
Cryo-EM structure of the persulfide-bound metallopterin-binding domain of molybdoenzyme ergothioneine synthase from Caldithrix abyssi
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 797453 |
| Software | cryoSPARC,PHENIX,SerialEM |
| CTF correction | PHASE FLIPPING ONLY |
