32JH

Nanobody Nb7 against SOG1 NAC domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-01-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	115.017, 67.656, 55.505
Unit cell angles	90.00, 90.59, 90.00

Refinement procedure

Resolution	40.100 - 2.450
R-factor	0.2656
Rwork	0.263
R-free	0.32820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.243
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	2.800
High resolution limit [Å]	2.450	2.450
Rmerge	0.186	0.522
Rmeas	0.211	0.586
Number of reflections	8645	1080
<I/σ(I)>	4.58	2.47
Completeness [%]	54.7	21
Redundancy	4.28
CC(1/2)	0.981	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Na-acetate pH 4.6 2.0 M (NH4)2SO4