32JG

Nanobody Nb3 against SOG1 NAC domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 2 21 21
Unit cell lengths	40.780, 66.480, 97.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.310 - 1.450
R-factor	0.1867
Rwork	0.185
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.778
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.310	1.540
High resolution limit [Å]	1.450	1.450
Rmerge	0.084	2.155
Rmeas	0.087	2.288
Number of reflections	46347	6246
<I/σ(I)>	13.63	0.72
Completeness [%]	96.9	82.3
Redundancy	12.59	8.69
CC(1/2)	0.999	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	0.1 M Na-citrate pH 5.6, 20% v/v 2-Propanol, 20% w/v PEG4000