32JE

Nanobody Nb1 against SOG1 NAC domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2026-02-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978564
Spacegroup name	P 21 21 21
Unit cell lengths	51.658, 92.152, 61.489
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 2.300
R-factor	0.2107
Rwork	0.207
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.942
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.060	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.255	1.163
Rmeas	0.281	1.286
Number of reflections	13474	2141
<I/σ(I)>	5.16	1.11
Completeness [%]	98.9	99.6
Redundancy	5.24	5.36
CC(1/2)	0.984	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.1 M Na-HEPES pH 7.5, 2% v/v PEG-400, 2.0 M (NH4)2SO4