32IM
Alpha-Synuclein Type 5A Fibrils at pH 7.0 with 2-start helical symmetry (co-existing with 1A, 1/7 and 2A fibrils in Sample 45)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 2.86 |
| Software | RELION,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
