32IB

Alpha-Synuclein Type 1D Fibrils at pH 7.4 formed by a 19:1 mixture of full-length protein and residues 5-140 (coexisting with 1m in sample 60)

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	HELICAL
Resolution (Å)	2.89
Software	RELION,EPU
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION