32EA
Horse spleen Holoferritin 14.5 MGy variable temperature dose series, 130 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 130 |
| Detector technology | CCD |
| Collection date | 2005-02-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9394 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.737, 181.737, 181.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 2.790 |
| R-factor | 0.17881 |
| Rwork | 0.177 |
| R-free | 0.22241 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.493 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.830 | 2.860 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmeas | 0.410 | 0.600 |
| Number of reflections | 6497 | 480 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 20 mg/ml protein, 0.6M (NH4)SO4, 10 mM CdSO4 Cryoprotection buffer was the mother liquor with 40% of the water replaced by glycerol |
