32DD
Horse spleen Holoferritin 17.4 MGy constant temperature dose series, 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9394 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.888, 181.888, 181.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.830 - 2.580 |
| R-factor | 0.19859 |
| Rwork | 0.197 |
| R-free | 0.23539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.830 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmeas | 0.160 | 0.600 |
| Number of reflections | 8160 | 606 |
| <I/σ(I)> | 11.2 | 0.7 |
| Completeness [%] | 99.6 | 99.84 |
| Redundancy | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 20 mg/ml protein, 0.6M (NH4)SO4, 10 mM CdSO4 Cryoprotection buffer was the mother liquor with 40% of the water replaced by glycerol |
