32DW

RAD52 N-terminal domain in complex with compound FORX-12-027

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.921240
Spacegroup name	P 21 21 21
Unit cell lengths	144.680, 147.230, 250.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.420 - 2.300
R-factor	0.18388
Rwork	0.182
R-free	0.21325
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	2.056
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	DIMPLE
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.420	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	236547	11587
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	0.999	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.05 M Sodium phosphate dibasic dihydrate, 5% w/v Polyethylene glycol 3,350