32DH

Crystal structure of YAP1(165-209) in complex with LATS2(511-522_C513S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.8856033
Spacegroup name	P 31 2 1
Unit cell lengths	53.036, 53.036, 37.593
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.090 - 1.030
R-factor	0.1868
Rwork	0.186
R-free	0.19190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.169
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	Aimless (v1.12.12)
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.930	1.120
High resolution limit [Å]	1.030	1.030
Rmerge	0.055	0.631
Number of reflections	23712	1186
<I/σ(I)>	19.3	1.9
Completeness [%]	94.3	63.4
Redundancy	13.8
CC(1/2)	0.999	0.796

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 ammonium acetate, 0.1 M Bis-Tris pH 6.5, and 25 % (w/v) PEG3350