328D
STRUCTURE OF A D(CGCGAATTCGCG)2-SN7167 COMPLEX
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-21 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.560, 40.150, 65.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BDL020 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.610 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.095 |
| Number of reflections | 1955 |
| Completeness [%] | 91.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MPD | 10 (%) | |
| 2 | 1 | drop | sodium cacodylate | 5.1 (mM) | |
| 3 | 1 | drop | SN7167 | 1 (mM) | |
| 4 | 1 | drop | 25.6 (mM) | ||
| 5 | 1 | drop | spermine hydrochloride | 0.4 (mM) | |
| 6 | 1 | drop | dodecamer | 1 (mM) | |
| 7 | 1 | reservoir | MPD | 50 (%) |
