327D
CRYSTAL STRUCTURES OF D(GM5CGM5CGCGCGC)
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06-01 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 39.330, 39.330, 77.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.940 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.22700 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.080 |
| Data reduction software | MSC |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.940 |
| Rmerge | 0.052 |
| Total number of observations | 12315 * |
| Number of reflections | 2601 |
| Completeness [%] | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 * | 22 * | VAPOR DIFFUSION |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 62 (mM) | |
| 2 | 1 | drop | DNA | 1 (mM) | single strand |
| 3 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 4 | 1 | drop | ammonium acetate | 12 (mM) | |
| 5 | 1 | reservoir | MPD | 75 (%(v/v)) |
