322D
CRYSTAL STRUCTURES OF D(CCGGGCCM5CGG)-HEXAGONAL FORM
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-04-01 |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 61 |
| Unit cell lengths | 55.030, 55.030, 45.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.19000 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.760 |
| Data reduction software | XENGEN (V. 2.0) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.036 |
| Number of reflections | 1954 |
| <I/σ(I)> | 9 |
| Completeness [%] | 71.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 22 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | single stranded |
| 2 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 5 | 1 | reservoir | MPD | 50 (%(v/v)) |
