31HY
Mycobacterium tuberculosis transcription termination factor Rho in complex with an RNA substrate bound to the secondary binding site and the ATP analogue ADP-BeF3
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Software | cryoSPARC,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
