31HE
Xray crystal structure of PtsM receptor binding protein
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.590, 67.370, 51.890 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 46.789 - 1.420 |
| Rwork | 0.185 |
| R-free | 0.20170 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.881 |
| Data reduction software | FAST_DP |
| Data scaling software | FAST_DP |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.810 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 103206 | 5146 |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.5 | |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M MgCl2, 0.1 M Na HEPES pH 7.5, 10% w/v PEG 4000 |
