31CT

Structure of recombinant Thermoascus aurantiacus xylanase TaXyn10 with an N-terminal pyroglutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-04
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	45.533, 58.351, 50.706
Unit cell angles	90.00, 110.79, 90.00

Refinement procedure

Resolution	24.859 - 1.110
Rwork	0.154
R-free	0.17140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.866
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.570	1.130
High resolution limit [Å]	1.110	1.110
Number of reflections	97755	4796
<I/σ(I)>	9	1.9
Completeness [%]	99.9
Redundancy	6.6
CC(1/2)	0.996	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	100 mM MES pH 6.7, 1.2 M ammonium sulfate