31AP

Crystal Structure of Bundibugyo Virus VP40 Dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	65.317, 91.632, 48.931
Unit cell angles	90.00, 97.56, 90.00

Refinement procedure

Resolution	33.310 - 1.630
R-factor	0.2005
Rwork	0.199
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.310	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	37456	3733
<I/σ(I)>	14.59	3.47
Completeness [%]	99.5
Redundancy	7
CC(1/2)	0.990	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25 % v/v ethylene glycol