314D
Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE WOBBLE BASE PAIR
Experimental procedure
Source type | SEALED TUBE |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1996-05-01 |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 20.280, 29.410, 51.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 14.000 * |
High resolution limit [Å] | 1.900 |
Rmerge | 0.072 |
Total number of observations | 5784 * |
Number of reflections | 2601 * |
Completeness [%] | 76.3 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | pH 7.00, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | MGCL2 | ||
5 | 1 | 1 | [CO(NH3)6]3+ | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | WATER |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 0.5 (mM) | single stranded |
2 | 1 | drop | sodium cacodylate | 50 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | cobalt hexammine | 2.5 (mM) | |
5 | 1 | drop | MPD | 5 (%(v/v)) | |
6 | 1 | reservoir | 17 (%) |