310D
Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1995-05-14 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 26.270, 26.270, 160.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 1.300 |
R-factor | 0.163 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ARN035 |
RMSD bond length | 0.025 |
RMSD bond angle | 0.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-96 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 8.000 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.070 | 0.444 |
Total number of observations | 96765 * | |
Number of reflections | 8165 | |
<I/σ(I)> | 32 | 3.2 |
Completeness [%] | 95.3 | 91.9 |
Redundancy | 11 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 5 (mg/ml) | |
2 | 1 | drop | HEPES | 50 (mM) | |
3 | 1 | drop | 15 (mM) | precipitant | |
4 | 1 | drop | spermine tetrahydrochloride | 1 (mM) | precipitant |
5 | 1 | drop | MPD | 30-40 (%) |