310D
Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 26.270, 26.270, 160.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.300 |
| R-factor | 0.163 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ARN035 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-96 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.070 | 0.444 |
| Total number of observations | 96765 * | |
| Number of reflections | 8165 | |
| <I/σ(I)> | 32 | 3.2 |
| Completeness [%] | 95.3 | 91.9 |
| Redundancy | 11 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | 15 (mM) | precipitant | |
| 4 | 1 | drop | spermine tetrahydrochloride | 1 (mM) | precipitant |
| 5 | 1 | drop | MPD | 30-40 (%) |
