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310D

Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA

Experimental procedure
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineBW7B
Temperature [K]277
Detector technologyIMAGE PLATE
Collection date1995-05-14
DetectorMARRESEARCH
Spacegroup nameP 61 2 2
Unit cell lengths26.270, 26.270, 160.900
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution8.000 - 1.300
R-factor0.163

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)ARN035
RMSD bond length0.025
RMSD bond angle0.000
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareSHELXL-96
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]8.0001.320
High resolution limit [Å]1.3001.300
Rmerge0.0700.444
Total number of observations96765

*

Number of reflections8165
<I/σ(I)>323.2
Completeness [%]95.391.9
Redundancy113.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.5

*

20

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropRNA5 (mg/ml)
21dropHEPES50 (mM)
31drop15 (mM)precipitant
41dropspermine tetrahydrochloride1 (mM)precipitant
51dropMPD30-40 (%)

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