30RF

Crystal structure of the asgard SNARE-like domain from Candidatus Heimdallarchaeota archaeon B3-JM-08

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.99999
Spacegroup name	I 4 2 2
Unit cell lengths	34.193, 34.193, 260.427
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.550 - 2.400
R-factor	0.2617
Rwork	0.261
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.336
Data reduction software	XDS (Jun 30, 3023 BUILT=20230630)
Data scaling software	XDS (Jun 30, 3023 BUILT=20230630)
Phasing software	PHENIX (1.20.1-4487)
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.550
High resolution limit [Å]	2.400	2.400
Number of reflections	5795	930
<I/σ(I)>	21.81	6.9
Completeness [%]	99.9	99.9
Redundancy	11.5	11.7
CC(1/2)	0.999	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293.15	H6 BCS