30RB

E. coli 70S ribosome with peptidyl-tRNA and Hsp15

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS GLACIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Energy Filter	TFS Selectris
Detector	TFS FALCON 4i (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.92
Number of Particle	3125488
Software	cryoSPARC,Servalcat,EPU,Coot
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION