30PP
Structure of a TrkB agonist bound to its TrkB epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-28 |
| Detector | DECTRIS EIGER2 XE CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 110.963, 110.963, 184.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.590 - 2.060 |
| R-factor | 0.2156 |
| Rwork | 0.214 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.611 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.590 | 2.130 |
| High resolution limit [Å] | 2.056 | 2.056 |
| Rpim | 0.027 | 0.357 |
| Number of reflections | 42061 | 4109 |
| <I/σ(I)> | 11.8 | 0.59 |
| Completeness [%] | 99.7 | 99.47 |
| Redundancy | 75.9 | 78.6 |
| CC(1/2) | 1.000 | 0.316 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG8k, 0.2M MgCl2, 0.1M Tris pH 8.5 |
