30PL

Putative Polyurethane Degrading Amidase Bound to Fragment VT00136

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-06-13
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.7293
Spacegroup name	P 61 2 2
Unit cell lengths	111.100, 111.100, 435.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.730 - 1.600
R-factor	0.28
Rwork	0.279
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	1.620
High resolution limit [Å]	1.600	1.600
Number of reflections	209563	15067
<I/σ(I)>	28.21
Completeness [%]	99.9
Redundancy	20
CC(1/2)	1.000	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% w/v PEG 3,350; 0.2 M Magnesium Acetate Tetrahydrate