30KY
Complex of Colletotrichum higginsianum effector protein ChEC108 with HMA domain 2 (HMA2) of HIPP6 from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.953738 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.701, 60.111, 77.118 |
| Unit cell angles | 70.36, 89.72, 84.01 |
Refinement procedure
| Resolution | 72.600 - 2.200 |
| R-factor | 0.17434 |
| Rwork | 0.172 |
| R-free | 0.22089 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.912 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.610 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.114 | 0.852 |
| Rmeas | 0.124 | 0.970 |
| Rpim | 0.049 | 0.449 |
| Total number of observations | 193959 | 11676 |
| Number of reflections | 32007 | 2659 |
| <I/σ(I)> | 9 | 1.3 |
| Completeness [%] | 99.4 | |
| Redundancy | 6.1 | 4.4 |
| CC(1/2) | 0.996 | 0.748 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16% PEG 6K, 0.1 M TRIS |
