30JU
X-ray structure of lysozyme treated with V(V)-lactate complex (structure B)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-14 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.225, 78.225, 37.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.310 - 1.355 |
| Rwork | 0.174 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.920 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.310 | 1.370 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Number of reflections | 25659 | 1243 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11.8 | |
| CC(1/2) | 0.998 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 20% ethylene glycol, 0.6 M sodium nitrate, 0.1 M sodium acetate pH 4.0 |
