30GJ

W-formate dehydrogenase from Nitratidesulfovibrio vulgaris (Desulfovibrio vulgaris) - Ambient temperature, Serial crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2025-03-08
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	65.500, 129.600, 151.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.787 - 1.930
Rwork	0.185
R-free	0.22240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.504
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.787	2.010
High resolution limit [Å]	1.930	1.930
Number of reflections	102629	10129
<I/σ(I)>	2.99	1.02
Completeness [%]	100.0	100
Redundancy	73.2	50.3
CC(1/2)	0.915	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8	293	32% PEG 3350, 0.1M Tris-HCl pH 8.0, 1M LiCl