30ES
Cryo-EM structure of the yeast RNA polymerase II elongation complex with 19-mer RNA in State V (TL-closed), in the presence of substrate ATP
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.54 |
| Software | cryoSPARC,UCSF Chimera,UCSF ChimeraX,PHENIX |
| CTF correction | NONE |
