30DB

Hen egg white lysozyme complexed with PEG, ACT, and CL (in meso crystallization)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.95373
Spacegroup name	P 43 21 2
Unit cell lengths	77.563, 77.563, 38.624
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.690 - 1.740
R-factor	0.1793
Rwork	0.177
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.787
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.850
High resolution limit [Å]	1.740	1.740
Rmerge	0.149
Rmeas	0.161
Number of reflections	12471	1954
<I/σ(I)>	9.98
Completeness [%]	99.4
Redundancy	7.1
CC(1/2)	0.997	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	600 mM sodium chloride, 100 mM sodium acetate pH 4.5 at 293 K, 30 %(v/v) PEG 400