30BC

Structure of the Hepatitis C Virus E2 Core from Genotype 6a in Complex with Germline Reverted Variants of the Broadly Neutralizing Antibody AR3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-09
Detector	DECTRIS PILATUS4 X 4M
Wavelength(s)	0.96546
Spacegroup name	P 21 21 21
Unit cell lengths	61.428, 66.952, 218.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.980 - 2.040
R-factor	0.2372
Rwork	0.235
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.308
Data reduction software	EDNA
Data scaling software	EDNA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.980	2.110
High resolution limit [Å]	2.040	2.080
Number of reflections	57518	2120
<I/σ(I)>	7.2
Completeness [%]	98.8
Redundancy	4
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium sulfate and 20% (w/v) PEG 3350