30AF
Complex between IgE-Fc and anti-IgE Fab aeFab98
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.356, 92.989, 105.077 |
| Unit cell angles | 90.00, 108.87, 90.00 |
Refinement procedure
| Resolution | 79.680 - 2.400 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.656 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.680 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.898 |
| Rmeas | 0.087 | 1.062 |
| Rpim | 0.046 | 0.559 |
| Total number of observations | 202389 | 15677 |
| Number of reflections | 57518 | 4475 |
| <I/σ(I)> | 10.8 | 1.3 |
| Completeness [%] | 98.8 | |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.995 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M sodium cacodylate pH 6.5, 8% (w/v) PEG 8000 and 0.1 M calcium acetate |
