Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 153 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.450, 34.660, 32.170 |
| Unit cell angles | 90.00, 113.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 0.031 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.600 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 1768 | |
| Completeness [%] | 75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | magnesium acetate | 30 (mM) | |
| 3 | 1 | drop | spermine | 1 (M) | |
| 4 | 1 | drop | MPD | 10 (%(v/v)) | |
| 5 | 1 | reservoir | MPD | 15 (%) |
