308D
BINDING OF DAUNOMYCIN TO B-D-GLUCOSYLATED DNA FOUND IN PROTOZOA TRYPANOSOMA BRUCEI STUDIED BY X-RAY CRYSTALLOGRAPH
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08-26 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 |
| Unit cell lengths | 18.619, 20.096, 26.553 |
| Unit cell angles | 69.09, 90.40, 108.03 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DDF001 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.100 |
| Data reduction software | R-AXIS |
| Phasing software | ULTIMA |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.096 |
| Number of reflections | 4688 |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | pH 6.00, VAPOR DIFFUSION, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 1 | SPERMINE | ||
| 6 | 1 | 1 | DIOXANE | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA hezamer | 1.2 (mM) | single stranded |
| 2 | 1 | drop | 4 (mM) | ||
| 3 | 1 | drop | sodium cacodylate | 35 (mM) | |
| 4 | 1 | drop | spermine | 2.5 (mM) | |
| 5 | 1 | drop | drug | 1.2 (mM) | |
| 6 | 1 | drop | dioxane | 0.02 (%) | |
| 7 | 1 | drop | MPD | 5 (%(v/v)) | |
| 8 | 1 | reservoir | 50 (%) |
