305D
SIDE-BY-SIDE BINDING OF DISTAMYCIN MOLECULES TO D(ICATATIC) IN THE TETRAGONAL FORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-03-24 |
| Detector | SIEMENS-NICOLET |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 27.860, 27.860, 58.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.170 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| Structure solution method | DIRECT REFINEMENT |
| Starting model (for MR) | GDLB51 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.400 |
| Data reduction software | XENGEN (2.0) |
| Data scaling software | XENGEN (V. 2.0) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.170 |
| Rmerge | 0.056 * |
| Number of reflections | 1432 |
| Completeness [%] | 83.0 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | pH 7.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 1 | SPERMINE_HCL | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | single strand concentration |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | distamycin hydrochloride | 1 (mM) | |
| 4 | 1 | drop | magnesium chloride | 20 (mM) | |
| 5 | 1 | drop | spermine hydrochloride | 1 (mg/ml) | |
| 6 | 1 | drop | MPD | 3 (%(v/v)) | |
| 7 | 1 | reservoir | 50 (%) |
