301D
CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MG(II)-SOAKED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-20 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 65.900, 65.900, 136.770 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.29700 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.800 |
High resolution limit [Å] | 3.100 |
Rmerge | 0.125 |
Number of reflections | 6561 |
<I/σ(I)> | 12.5 |
Completeness [%] | 99.1 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | pH 8.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | LI SULFATE | ||
3 | 1 | 1 | MG SULFATE | ||
4 | 1 | 1 | EDTA | ||
5 | 1 | 1 | NA CACODYLATE |