2ZZT

Crystal structure of the cytosolic domain of the cation diffusion facilitator family protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97923, 0.97939, 0.96416
Spacegroup name	H 3 2
Unit cell lengths	97.719, 97.719, 83.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.863 - 2.842
R-factor	0.236
Rwork	0.235
R-free	0.25700
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.475
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.840	2.840
Number of reflections	3707
<I/σ(I)>	32.5	5.6
Completeness [%]	99.4	98.9
Redundancy	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6-2.0M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K