2ZZT
Crystal structure of the cytosolic domain of the cation diffusion facilitator family protein
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97923, 0.97939, 0.96416 |
Spacegroup name | H 3 2 |
Unit cell lengths | 97.719, 97.719, 83.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.863 - 2.842 |
R-factor | 0.236 |
Rwork | 0.235 |
R-free | 0.25700 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.890 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 3707 | |
<I/σ(I)> | 32.5 | 5.6 |
Completeness [%] | 99.4 | 98.9 |
Redundancy | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6-2.0M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |